Dr. Lester Khoo

Dr. Lester Khoo

Department

  • Pathobiology and Population Medicine
  • Delta R&E Center
  • MAFES
  • Thad Cochran National Warmwater Aquaculture Center

Title

  • Professor & Director

Contacts

lhk2@msstate.edu
Office: (662) 686-3305
189 TCNWAC

Overview

Education:

  • Doctor of Philosophy (Ph.D.), Immunology, North Carolina State University—Raleigh, 1996
  • Doctor of Veterinary Medicine (D.V.M.), Veterinary Medicine, University Of Pennsylvania, 1990
  • Bachelor of Science (B.S.), Biological Sciences, University of Guelph, 1986

Research interests:

Infectious and non infectious aquatic diseases

Publications

  • Iron supplementation to promote red blood cell production in anemic catfish. 2013 DREC Annual Report, p. 30. 2014

Book

  • Infestations of the trematode Bolbophorus sp. in channel catfish. Southern Regional Aquaculture Center. 2003

Book, Chapter

  • Henneguya ictaluri. Fish parasites: pathobiology and protection. Cambridge, MA: CABI. 2011

Conference Proceeding

  • Using 1-D and 2-D-J-Resolved 1H-NMR metabolomics to understand the effects of anemia in channel catfish, Ictalurus punctatus. .  Aquaculture America. 2015

DREC Annual Report

  • Overview of NWAC Fish Disease Research Program. Delta Research and Extension Center Annual Report 2017. Pages 46-47. 2018
  • Biomphalaria obstructa (syn. B. havanensis) is host to two species of trematodes infective to catfish. . Delta Research and Extension Center. 2016
  • Characterization of myxozoan actinospore from a commercial catfish pond in the Mississippi delta.. Delta Research and Extension Center. 2016
  • Edwardsiella piscicida: A new catfish pathogen.. Delta Research and Extension Center. 2016

DREC annual report

  • Development of an oral vaccination platform to protect catfish against enteric septicemia of catfish. DREC Annual report 2017. 2018

Journal Article

  • New data on Henneguya postexilis Minchew, 1977, a parasite of channel catfish Ictalurus punctatus, with notes on resolution of molecular markers for myxozoan phylogeny. Systematic Parasitology. 2022
  • An orally delivered, live-attenuated Edwardsiella ictaluri vaccine efficiently protects channel catfish fingerlings against multiple Edwardsiella ictaluri field isolates. Journal of the World Aquaculture Society. Volume 51, Issue 6, Pages 1354-1372. 2020
  • Validation of Edwardsiella ictaluri oral vaccination platform in experimental pond trialsJournal of the World Aquaculture Society. Volume 51, Issue 2, Pages 346-363. 2020
  • Assessment of Bolbophorus damnificus prevalence and cercariae shedding in Planorbella trivolvis populations from catfish aquaculture ponds in Mississippi, USAJournal of the World Aquaculture Society. 2020
  • Effect of understocking density of channel catfish fingerlings in intensively aerated multiple-batch productionJournal of the World Aquaculture Society. 2020
  • Arrested development of Henneguya ictaluri (Cnidaria: Myxobolidae) in♀ channel catfish$\times$♂ blue catfish hybrids. Journal of aquatic animal health. Volume 31, Issue 2, Pages 201--213. 2019
  • Pathologic changes associated with respiratory compromise and morbidity due to massive interlamellar Henneguya exilis infection in channel$\times$ blue hybrid catfish. Journal of Parasitology. Volume 105, Issue 5, Pages 686--692. 2019
  • Emergence of Edwardsiella piscicida in farmed Channel ♀, Ictalurus punctatus × Blue ♂, Ictalurus furcatus, hybrid catfish cultured in MississippiJournal of the World Aquaculture Society. Volume 50 , Issue 2, Pages 420-432. 2019
  • Performance of Channel catfish and Hybrid catfish in single‐batch, intensively aerated pondsNorth American Journal of Aquaculture. Volume 81, Issue 4, Pages 406-416. 2019
  • Economic assessment of commercial-scale Edwardsiella ictaluri vaccine trials in U.S. catfish industryAquaculture Economics & Management. Volume 23, Issue 3, Pages 254-275. 2019
  • Arrested Development of Henneguya ictaluri (Cnidaria: Myxobolidae) in ♀ Channel Catfish × ♂ Blue Catfish HybridsJournal of Aquatic Animal Health. Volume 31, Issue 2, Pages 201-213. 2019
  • Arrested development of Henneguya ictaluri (Cnidaria: Myxobolidae) in channel catfish Ictalurus punctatus, blue catfish Ictalurus furcatus, and their hybrid cross. Journal of Aquatic Animal Health. Volume 31, Pages 201-213. 2019
  • Comparative susceptibility of channel catfish, ictalurus punctatus; blue catfish, ictalurus furcatus; and channel (♀)$\times$ blue (♂) hybrid catfish to Edwardsiella piscicida, Edwardsiella tarda, and Edwardsiella anguillarum. Journal of the World Aquaculture Society. Volume 49, Issue 1, Pages 197--204. 2018
  • Encapsulation of Bolbophorus damnificus (Digenea: Bolbophoridae) metacercariae in juvenile channel catfish Ictalurus punctatus is linked to delayed onset mortality. Journal of the World Aquaculture Society. Volume 49, Issue 3, Pages 601-611. 2018
  • Encapsulation of Bolbophorus damnificus (Digenea: Bolbophoridae) Metacercariae in Juvenile Channel Catfish, Ictalurus punctatus, Is Linked to Delayed-onset MortalityJournal of the World Aquaculture Society. Volume 49, Issue 3, Pages 601-611. 2018
  • Comparative Susceptibility of Channel Catfish, Ictalurus punctatus; Blue Catfish, Ictalurus furcatus; and Channel (♀) × Blue (♂) Hybrid Catfish to Edwardsiella piscicida, Edwardsiella tarda, and Edwardsiella anguillarum. . Journal of the World Aquaculture Society. Volume 49(1), Pages 197-204. 2018
  • Mortality associated with acute development of Bolbophorus damnificus (Digenea: Bolbophoridae) in juvenile channel catfish Ictalurus punctatus.. Journal of World Aquaculture Society. 2017
  • North and South American haplotypes of Drepanocephalus auritus (Digenea: Echinostomatidae) are released from Biomphalaria havanensis (Mollusca: Planorbidae) inhabiting catfish aquaculture ponds in Mississippi, USA. Comparative Parasitology. Volume 84, Issue 2, Pages 87--101. 2017
  • Comparative phenotypic and genotypic analysis of Edwardsiella isolates from different hosts and geographic origins, with emphasis on isolates formerly classified as E. tarda, and evaluation of diagnostic methods. Journal of Clinical Microbiology. Volume 55, Issue 12, Pages 3466-3491. 2017
  • North and South American Haplotypes of Drepanocephalus auritus (Digenea: Echinostomatidae) Are Released from Biomphalaria havanensis (Mollusca: Planorbidae) Inhabiting Catfish Aquaculture Ponds in Mississippi, U.S.A.Comparative Parasitology. Volume 84, Issue 2, Pages 87-101. 2017
  • Biomphalaria havanensis is a natural first intermediate host for the trematode Bolbophorus damnificus in commercial catfish production in Mississippi. North American Journal of Aquaculture. Volume 78, Issue 3, Pages 189--192. 2016
  • Characterization of the life cycle of a fish eye fluke, Austrodiplostomum ostrowskiae (Digenea: Diplostomidae), with notes on two other diplostomids infecting Biomphalaria havanensis (Mollusca: Planorbidae) from catfish aquaculture ponds in Mississippi, USA. Journal of Parasitology. Volume 102, Issue 2, Pages 260--274. 2016
  • Verrucous dermal henneguyosis associated with Henneguya exilis (Kudo, 1929) (Cnidaria: Myxobolidae), a parasite of the channel catfish Ictalurus punctatus (Rafinesque, 1818)Journal of fish diseases. Volume 39, Issue 10, Pages 1263-1267. 2016
  • Biomphalaria havanensis is a Natural First Intermediate Host for the Trematode Bolbophorus damnificus in Commercial Catfish Production in MississippiNorth American Journal of Aquaculture. Volume 78, Issue 3, Pages 189-192. 2016
  • Characterization of the Life Cycle of a Fish Eye Fluke, Austrodiplostomum ostrowskiae (Digenea: Diplostomidae), with Notes on Two Other Diplostomids Infecting Biomphalaria havanensis (Mollusca: Planorbidae) from Catfish Aquaculture Ponds in Mississippi, USAJournal of Parasitology. Volume 102, Issue 2, Pages 260-274. 2016
  • Using 1-D 1 H and 2-D 1 H J-resolved NMR metabolomics to understand the effects of anemia in channel catfish (Ictalurus punctatus). Metabolomics. Volume 11, Issue 5, Pages 1131-1143. 2015
  • Oral Vaccination of Channel Catfish against Enteric Septicemia of Catfish Using a Live Attenuated Edwardsiella ictaluri Isolate.. Journal of aquatic animal health. Volume 27, Issue 2, Pages 135-43. 2015
  • Myxobolus neurophilus Guilford 1963 (Myxosporea: Myxobolidae): a common parasite infecting yellow perch Perca flavescens (Mitchell, 1814) in Saskatchewan, Canada.. Journal of fish diseases. Volume 38, Issue 4, Pages 355-64. 2015
  • Small subunit ribosomal RNA sequence links the myxospore stage of Henneguya mississippiensis n. sp. from channel catfish Ictalurus punctatus to an actinospore released by the benthic oligochaete Dero digitata.. Parasitology research. Volume 114, Issue 4, Pages 1595-602. 2015
  • Small subunit ribosomal RNA sequence links the myxospore stage of Henneguya mississippiensis n. sp. from channel catfish Ictalurus punctatus to an actinospore released by the benthic oligochaete Dero digitataParasitology Research. Volume 114, Issue 4, Pages 1595-1602. 2015
  • Myxobolus neurophilus Guilford (Myxosporea: Myxobolidae): a common parasite infecting yellow perch Perca flavacens (Mitchell, 1814) in Saskatchewan, Canada. J Fish Dis. 2014
  • 18S rRNA gene sequencing identifies a novel species of Henneguya parasitizing the gills of the channel catfish (Ictaluridae).. Parasitology research. Volume 113, Issue 12, Pages 4651-8. 2014
  • Chronic pathology and longevity of Drepanocephalus spathans infections in juvenile Channel Catfish.. Journal of aquatic animal health. Volume 26, Issue 4, Pages 210-8. 2014
  • Molecular and morphological characterization of myxozoan actinospore types from a commercial catfish pond in the Mississippi delta.. The Journal of parasitology. Volume 100, Issue 6, Pages 828-39. 2014
  • Comparative susceptibility of Channel Catfish, Blue Catfish, and their hybrid cross to experimental challenge with Bolbophorus damnificus (Digenea: Bolbophoridae) cercariae.. Journal of aquatic animal health. Volume 26, Issue 2, Pages 96-9. 2014
  • Lactococcosis in Silver Carp.. Journal of aquatic animal health. Volume 26, Issue 1, Pages 1-8. 2014
  • Edwardsiella piscicida identified in the Southeastern USA by gyrB sequence, species-specific and repetitive sequence-mediated PCR.. Diseases of aquatic organisms. Volume 108, Issue 1, Pages 23-35. 2014
  • Iron status of channel catfish Ictalurus punctatus affected by channel catfish anemia and response to parenteral iron.. Diseases of aquatic organisms. Volume 107, Issue 3, Pages 241-8. 2014
  • 18S rRNA gene sequencing identifies a novel species of Henneguya parasitizing the gills of the channel catfish (Ictaluridae)Parasitology Research. Volume 113, Issue 12, Pages 4651-4658. 2014
  • Molecular and morphological characterization of myxozoan actinospore types from a commercial catfish pond in the Mississippi deltaJournal of Parasitology. Volume 100, Issue 6, Pages 828-839. 2014
  • Impacts of Bolbophorus damnificus (Digenea: Bolbophoridae) on Production Characteristics of Channel Catfish, Ictalurus punctatus, Raised in Experimental Ponds. Journal of the World Aquaculture Society. Volume 44, Issue 4, Page 557–564. 2013
  • Palatability of diets for channel catfish that contain Amprolium or Salinomycin using feed conversion ratio as the criterion. North American Journal of Aquaculture. Volume 75, Issue 1, Page 99–101. 2013
  • Complete Genome Sequence of a Channel Catfish Epidemic Isolate, Aeromonas hydrophila Strain ML09-119.. Genome announcements. Volume 1, Issue 5. 2013
  • Genetic sequence data identifies the cercaria of Drepanocephalus spathans (Digenea: Echinostomatidae), a parasite of the double-crested cormorant (Phalacrocorax auritus), with notes on its pathology in juvenile channel catfish (Ictalurus punctatus).. The Journal of parasitology. Volume 98, Issue 5, Pages 967-72. 2012
  • Ameliorating the effects of the digenetic trematode, Bolbophorus damnificus on the channel catfish industry. . 2011
  • A real-time polymerase chain reaction assay for quantification of Edwardsiella ictaluri in catfish pond water and genetic homogeneity of diagnostic case isolates from Mississippi.. Journal of aquatic animal health. Volume 23, Issue 4, Pages 178-88. 2011
  • The pathology associated with visceral toxicosis of catfish.. Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. Volume 23, Issue 6, Pages 1217-21. 2011
  • A duplex real-time polymerase chain reaction assay for differentiation between Bolbophorus damnificus and Bolbophorus type II species cercariae.. Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. Volume 22, Issue 4, Pages 615-22. 2010
  • Myxobolus neurophilus: morphologic, histopathologic and molecular characterization.. Diseases of aquatic organisms. Volume 89, Issue 1, Pages 51-61. 2010
  • Effects of dried algae Schizochytrium sp., a rich source of docosahexaenoic acid, on growth, fatty acid composition, and sensory quality of channel catfish Ictalurus punctatus. Aquaculture. Volume 292, Issue 3, Page 232–236. 2009
  • New data on Henneguya pellis (Myxozoa: Myxobolidae), a parasite of blue catfish Ictalurus furcatus.. The Journal of parasitology. Volume 95, Issue 6, Pages 1455-67. 2009
  • Induction and evaluation of proliferative gill disease in channel catfish fingerlings.. Journal of aquatic animal health. Volume 20, Issue 4, Pages 236-44. 2008
  • The Epidemiology of Bacterial Diseases in Food-Size Channel Catfish.. Journal of aquatic animal health. Volume 18, Issue 4, Pages 263-72. 2006
  • Evaluation of channel catfish virus latency on fingerling production farms in Mississippi. Journal of Aquatic Animal Health. Volume 17, Issue 3, Page 211–215. 2005
  • Bolbophorus confusus infections in channel catfish in northwestern Mississippi and effects of water temperature on emergence of cercariae from infected snails. North American Journal of Aquaculture. Volume 64, Issue 1, Page 70–74. 2002
  • The epidemiology of bacterial diseases in food-size channel catfish. Journal of Aquatic Animal Health. Volume 14, Issue 4, Page 263–272. 2002

NWAC News

  • Development and use of primary fish cell cultures in fish disease diagnosis. . 2017
  • Development of an oral vaccination platform to protect catfish against Enteric Septicemia of Catfish.. 2017
  • Encapsulation of trematode larvae metacercariae is linked to mortality in channel catfish. . 2017
  • Evaluation of factors potentially affecting vaccine safety and efficacy. . 2017

NWAC article

Published Abstract

  • Fish host susceptibility influences myxozoan community composition of proliferative gill disease in catfish aquaculture. Annual Meeting of the American College of Veterinary Pathologists. 2020
  • Fish host susceptibility influences myxozoan community composition of Proliferative Gill Disease in catfish aquaculture.. Steeve Giguere Science of Veterinary Medicine Symposium . 2020
  • Fish host susceptibility influences myxozoan community composition of proliferative gill disease in catfish aquaculture. American Fisheries Society: Fish Health Section Summer Seminar Series .
  • Fish host susceptibility influences myxozoan community composition and diversity in proliferative gill diseases of catfish aquaculture. Annual Meeting of the American Association of Veterinary Pathologist . 2020
  • Genomic analysis of atypical Aeromonas hydrophila (aAh) from catfish aquaculture in the southeastern United States with evidence of haplotype shifts from diagnostic case submissions. . AFS-FHS Virtual Summer Seminar Series. 2020

Trade Journal/Magazine

  • Avoiding Residues in U.S. Farm-raised Catfish. NWAC Newletter. Volume 14, Issue 1, Page 24. 2017

Presentations

Abstract

  • "Expanding our knowledge of the Edwardsiella. ." American Society Microbiology, , Boston, MA. 2016
  • "Unraveling myxozoan parasite diversity in catfish aquaculture in the southeastern United States. ." American Society Microbiology, , Boston, MA. 2016
  • "Comparison of Edwardsiella ictaluri isolates from different hosts and geographic origins." Food Security for the Future: The Role of Aquatic Animal Health, , Starkville, MS. 2015
  • "An overview of myxozoan parasites associated with catfish aquaculture." 40th Annual Eastern Fish Health Workshop, American Fisheries Society, Charleston, South Carolina. 2015
  • "Recent advancements in our knowledge of Edwardsiella piscicida and E. tarda." 40th Annual Eastern Fish Health Workshop, American Fisheries Society, Charleston, South Carolina. 2015
  • "Using 1-D and 2-D J-Resolved 1H-NMR metabolomics to understand the effects of anemia in channel catfish, Ictalurus punctatus.." Aquaculture America, World Aquaculture Society, New Orleans . 2015
  • "A team approach in the study and control of the digenetic trematode Bolbophorus damnificus in commercial catfish." Proceedings of the 7th International Symposium on Fish Parasites, International Symposium on Fish Parasites, Viterbo, Italy. 2007

Oral Presentation

  • "An abbreviated history of proliferative gill disease research: past and present." 45th Eastern Fish Disease Workshop, American Fisheries Society, Shepherdstown, WV. 2020
  • "Attempts at biological control of proliferative gill disease in catfish aquaculture." American Society of Parasitologists 2019 Annual Meeting, ASP, Rochester, MN. 2019
  • "Economic assessment of commercial-scale Edwardsiella ictaluri vaccine trials in the U.S. catfish industry." World Aquaculture Society, World Aquaculture Society, New orleans, LO, USA. 2019
  • "Edwardsiella piscicida, an emergent pathogen in farmed Channel ♀, Ictalurus punctatus x Blue ♂, Ictalurus furcatus Hybrid catfish cultured in Mississippi." 8th International Symposium on Aquatic Animal Health, American Fisheries Society Fish Health Section, Charlottetown, Prince Edward Island, Canada. 2018
  • "Fish Vaccination--Factors to Consider." International Symposium for Aquatic Animal Health, International symposium for Aquatic Animal Health, Prince Edward Island, Canada. 2018
  • "Monoculture of hybrid catfish can limit proliferative gill disease caused by Henneguya ictaluri (Myxozoa: Myxobolidae) in catfish aquaculture ponds." International Symposium for Aquatic Animal Health, International Symposium for Aquatic Animal Health, Prince Edward Island, Canada. 2018
  • "Hybrid catfish monoculture limits proliferative gill disease caused by Henneguya ictaluri (Myxozoa: Myxobolidae) in catfish aquaculture ponds.." 43rd Annual Eastern Fish Health Workshop, , Chattanooga, TN. 2018
  • "Emergence of Edwardsiella piscicida in farmed Channel ♀, Ictalurus punctatus x Blue ♂, Ictalurus furcatus Hybrid catfish cultured in Mississippi." 43rd Eastern Fish Health Workshop, Eastern Fish Health Workshop, Chattanooga, Tennessee . 2018
  • "Hybrid catfish monoculture can limit incidence and severity of proliferative gill disease caused by Henneguya ictaluri (Myxozoa: Myxobolidae) in catfish aquaculture.." Southeastern Society of Parasitologists, The American Society of Parasitologists, Starkville, MS. 2018
  • "Economic benefits of oral ESC vaccination." East Mississippi catfish symposium, mississippi state university, Macon MS. 2017
  • "An emerging disease in hybrid catfish. ." NWAC Fall Seminar Series, MSU, Macon,MS. 2017
  • "An emerging disease in hybrid catfish. ." NWAC Fall Seminar Series, MSU, Stoneville, and Macon MS. 2017
  • "Economic benefits of oral ESC vaccination." NWAC Mississippi catfish symposium, mississippi state university, stoneviille. 2017
  • "Sampling regimes and chemical usage in the catfish industry." Dr. Pat Basu, Chief Public Health Veterinarian – USDA FSIS , MSU Extension, Stoneville, MS. 2017
  • "Advancing our knowledge of the bacteria formerly known as Edwardsiella tarda.." 42nd Eastern Fish Health Workshop, , East Lansing, MI. 2017
  • "Arrested Development of Henneguya ictaluri (Cnidaria: Myxobolidae) In Channel (♀) x Blue (♂) Hybrid Catfish. ." 42nd Eastern Fish Health Workshop, , East Lansing, MI. 2017
  • "Evaluation of factors affecting the safety and efficacy of Edwardsiella ictaluri vaccine.." 42nd Eastern Fish Health Workshop, , East Lansing, MI. 2017
  • "Economic benefits of oral ESC vaccination." WAS, WAS, San Antonio Tx. 2017
  • "Mortality associated with acute development of Bolbophorus damnificus (Digenea: Bolbophoridae) in juvenile channel catfish Ictalurus punctatus.." 41 st Eastern Fish Health Workshop, , Atlantic Beach, NC. 2016
  • "Development of a risk assessment model for proliferative gill disease in channel catfish." Myxozoan Parasites of Fish Conference, Myxozoan Parasites of Fish Conference, Nanaimo, British Columbia. 2002

Areas of Expertise

Fish diseases pathology